In vitro radiosensitivity of human fresh T-lymphocytes by colony formation assay using PHA and recombinant interleukin-2.
نویسندگان
چکیده
Radiosensitivity/Ionizing radiation/Cell killing/Human T-lymphocytes In vitro culture conditions for colony formation of human fresh peripheral T-cells using PHA and recombinant Interleukin-2 are defined. Peripheral lymphocytes, from six individuals, were exposed to X or gamma rays in vitro, and dose-survival curves were obtained. The results showed typical sigmoid curves similar to those observed when other mammalian cells are exposed to radiation. The D10 (dose required to kill 90% of the cells) was found to be 3.0 to 3.5 Gy.
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ورودعنوان ژورنال:
- Journal of radiation research
دوره 28 3 شماره
صفحات -
تاریخ انتشار 1987